Antitumor activity of copper(II) complexes with phenanthroline and dipeptides in cultured breast cancer cells
Actividad antitumoral de complejos de cobre(II) con fenantrolina y dipéptidos en células de cáncer de mama
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The search for non-toxic anti-cancer therapies has increased, allowing the development of new metal complexes linked to essential molecules such as amino acids. For this reason, two copper(II) compounds were studied with a main ligand such as 1,10 phenanthroline (phen). The complex [CuCl2(phen)] (1) was studied in comparison to the ternary complex with a dipeptide (the result of the union of two amino acids such as alanine and phenylalanine as an auxiliary ligand) [Cu(L-Ala-Phe) (phen)]·4 H2O (2) in the human breast cancer cell line MCF-7. Functional analysis showed that both complexes induce antiproliferative and an effect on clone inhibition; Anyway, neither of the two complexes showed an increase in the production of EROs. On the other hand, the phenomenon of cell death induced by 1 occurs through the necrosis process, while 2 causes the induction of early apoptosis. Likewise, only at low concentrations of 2 (1 μM), we found double and single-strand DNA breaks, which suggests that it could be one of the molecular targets related to its antitumor activity. This compound is a promising candidate for further in vivo studies that prove to be a good alternative therapy for breast cancer.
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