EGF y VEGF levels in platelet-rich plasma before and after antiplatelet drugs.
Niveles de EGF y VEGF en el plasma rico en plaquetas antes y después de antiagregantes plaquetarios.
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Background: The platelet-rich plasma (PRP) is considered rich product autologous growth factors essential in tissue regeneration. Objective: to quantify the concentrations of VEGF and EGF in the PRP before and after platelet aggregation: aspirin (ASA) and clopidogrel; and its correlation with the platelet count. Material and methods: we studied in 32 healthy subjects, concentrations of EGF and VEGF (Elisa) in PRP, platelet-poor (PPP), exudate, lysate before and after of aspirin (AAS) and clopidogrel (CLO). Results: the concentration of EGF in the PRP and its products before and after treatment with AAS or clopidogrel, showed no significant difference for none of the analyzed platelet derivatives; not so for the VEGF where significant difference was found in the ‘lysate’ by-product (p<0.05). Appreciated correlation between platelet count in the basal PRP from healthy subjects (578.25±125.33x103xmm3) versus the baseline concentration of EGF in the group treated with ASA (296±203.6pg/ml) (r=0.726, p<0.01), but not for clopidogrel. Conclusions: platelet numbers, both by-products of concentrated platelet or peripheral blood, no are predictive of the concentrations of FCs, since other elements can influence the final release of them. Similarly the data seem to point the clinical utility that can take the implementation of PRP in subjects treated with ASA or clopidogrel, at the time that needs you so.
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